NanoString, mRNA, Dendritic Cells

 

 

ICM Research Seminar Programme

Speakers:  Kile Green & Anders Andreasson

 

Venue: Seminar room L2.5, 2^nd floor, William Leech Building, Medical School

Date and time:  Wednesday 28^th January 2015 at 12.30

 

Kile Green will present:

 

"NanoString Technology and in-silico testing of Dendritic Cell subset separation"

(Dr Venetia Bigley & Prof. Matt Collin: Human Dendritic Cell group)

 

NanoString is an emerging technology capable of high throughput expression analysis of up to 800 targets in a single reaction. Cartridges for this technology can be custom designed or purchased as pre-built kits. I designed an in-silico experiment to determine the efficiency of an immunology-based kit at categorising Dendritic Cell subsets from the skin and blood using surrogate Illumina expression data. An additional probeset was then designed to further separate these subsets.

 

 

Key words: NanoString, mRNA, Dendritic Cells

_____________________________

Anders Andreasson will discuss:

 

"Identifying a biosignature of transplantable and non-transplantable human donor lungs in clinical ex vivo lung perfusion – a feasibility study"

(Professor Andrew Fisher)

 

Availability of donor lungs suitable for transplantation falls short of current demand and contributes to significant waiting list mortality. Ex vivo lung perfusion (EVLP) offers opportunity to objectively assess and recondition organs unsuitable for immediate transplantation. We performed a study investigating putative molecular markers in bronchoalveolar lavage fluid (BALF) and perfusate that may differentiate transplantable from non-transplantable donor lungs during EVLP.

 

 

Key words: Lung transplantation, Ex vivo lung perfusion, Biosignature

 

 

Chair:  Sophie Cassidy