Tuesday 16 December 2014

Hepatocytes and Antioxidant Screening and Osteoarthritis Susceptibility



Institute Research Student Seminars

Speakers:  Emily Hudson & Katherine Johnson


Venue: Seminar room L2.5, 2nd floor, William Leech Building, Medical School

Date and time:  Wednesday 17th December 2014 at 12.30


Emily Hudson will present:


“Derivation of human hepatocytes from pancreatic progenitor cells and their use in a novel antioxidant screening platform”

(Diagnostic and Therapeutic Technology Group)


Primary hepatocytes are considered an ideal cell model for the study of hepatotoxicity, however they’re difficult to culture in vitro as they resist proliferation.  While there are a large variety of other models used to investigate liver toxicity there is yet to be a fully competent model that can be produced in required to meet demand.  A possible solution to this problem is the conversion of pancreatic cells to hepatocytes using steroid treatment.  This has already been shown to be viable in rodent cells with the creation of the B13/H cell line, derived from the B13 cell line.  When tested, if these B13/H cells respond to hepatotoxins in the same way as hepatocytes it could provide the rationale for expanding the research into a human cell line and ultimately generate a cell line for use in regulatory toxicology studies, disease modelling.



Key words: Reactive Oxygen Species, Hepatocytes, Nanosensors


Katherine Johnson will speak on:


Functional analysis of the osteoarthritis susceptibility locus marked by the polymorphism rs10492367”

(John Loughlin: Musculoskeletal Research)


The 2012 arcOGEN genome-wide association study reported that the rs10492367 G to T single nucleotide polymorphism (SNP) marks a region on chromosome 12p that is associated with hip osteoarthritis (OA) in Europeans. To functionally dissect this region, we i) investigated differential transcription factor binding, ii) quantified expression of nearby genes and iii) analysed methylation levels across the region.



Keywords: Osteoarthritis, functional analysis, methylation



Chair:  Sophie Cassidy







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